| 克隆类型 ： |
Mouse Monoclonal Antibody |
|
亚 型 ： |
mouse IgG1 |
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免 疫 原 ： |
? |
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产品描述 ： |
This antibody recognizes a polymorphism variant of CD16 alpha antigen, which is a low affinity receptor for aggregated IgG (Fc gamma receptor III). The receptor exists in two distinct forms: a glycosyl phosphatidyl inositol (GPI) form (Fc gamma RIIIB), and an integral membrane protein (Fc gamma RIIIA). In humans, the transmembrane form is expressed on NK cells and macrophages whereas the GPI-linked form is expressed on granulocytes. This antibody binds both forms but binds poorly to NK cells from donors not carrying the polymorphism. The antibody can be used for detecting CD16 in flow cytometry, immunoprecipitation, western blot and blocks ligand binding. |
| 适用物种 ： |
H |
|
应用范围 ： |
B, FC, IP, WB |
| 应用说明 ： |
FC with whole blood: 100 μl blood + 10 μl antibody; FC with cells: 500,000 cells in 50 μl + 10 μl antibody. This antibody recognizes a polymorphism in CD16a antigen, therefore binding poorly to NK cells from certain donors. The antibody blocks ligand binding. |
| 别 名 ： |
Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-2; Fc-gamma RIII-alpha; Fc-gamma RIIIa; FcRIIIa; Fc-gamma RIII; FcRIII; FcR-10; CD16a antigen; FCGR3A; CD16A; FCG3; FCGR3; IGFR3 |
|
形 态 ： |
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography. |
| 保存条件 ： |
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
|
产品文献 ： |
Tamm A. and Schmidt R. 1996. J. Immunology. 157: 1576-81. PMID# 8759741. |
| 产品链接 ： |
http://www.abbiotec.com/antibodies/cd16-alpha-antibody |
| 标 记 物 ： |
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产品说明 ： |
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