| 克隆类型 ： |
Mouse Monoclonal Antibody |
|
亚 型 ： |
Mouse IgG1/k |
|
免 疫 原 ： |
Pepsin-solubilized, salt-fractionated type I collagen from human placenta |
|
产品描述 ： |
Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Defects in one or both alleles of the type I collagen gene causes "brittle bone disease" (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones. This antibody is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). The antibody reacts with human, calf, sheep, pig, dog and goat type I collagens in ELISA and immunoblotting, but does not react with mouse, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen. The antibody has a Ka of approximately 2.5 x 106 M-1 for human type I collagen. |
| 适用物种 ： |
H |
|
应用范围 ： |
E, WB, IHC |
| 应用说明 ： |
Product Cat. No. 250274 can be used to detect collagens by ELISA. In immunoblotting Product Cat. No. 250274 detects human type I collagen only in its native triple helix form. Product Cat. No. 250274 can be used in immunohistochemistry with frozen unfixed sections of human and bovine skin. If tissue fixation is required, acetone or ethanol is recommended. E: 1:16,000; WB: 1:1,000; IHC: 1:200 |
| 别 名 ： |
Collagen alpha-1(I) chain; alpha-1 type I collagen; COL1A1 |
|
形 态 ： |
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件 ： |
Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
|
产品文献 ： |
Choi JS et al. 2014. Biomaterials. 40:98-106. PMID#25433606. |
| 产品链接 ： |
http://www.abbiotec.com/antibodies/collagen-type-i-5d8-antibody |
| 标 记 物 ： |
|
|
产品说明 ： |
|
|
