| 克隆类型 ： |
Rabbit Polyclonal IgG |
|
免 疫 原 ： |
Peptide derived from human ATP6V1B2. |
|
纯化方式 ： |
Immunogen affinity purified |
|
缓 冲 液 ： |
PBS, pH 7.4(1%BSA and 0.1% Sodium azide) |
|
产品描述 ： |
Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. |
| 适用物种 ： |
Hu, Mo, Rat, Chi, Dog, Pig, Cow, Hor, Sheep, Cynomolgus Monkey, Orangutan. |
| 应用说明 ： |
WB: 1:200-1:1000 E: 1:500-1:1000 IHC: 1:200-1:1000 IF: 1:100-1:500 |
| 别 名 ： |
V-ATPase B2; ATP6B1B2; ATP6B2; ATPase H+ transporting lysosomal 56/58kDa V1 subunit B isoform 2; ATPase H+ transporting lysosomal 56/58kDa V1 subunit B2; ATPase H+ transporting, lysosomal (vacuolar proton pump) beta polypeptide 56/58kD isoform 2; ATPase, H+ transporting, lysosomal V1 subunit B2; brain isoform; Endomembrane proton pump 58 kDa subunit; H+ transporting two sector ATPase; HO 57; HO57; V ATPase B2 subunit; V ATPase subunit B 2; V type proton ATPase subunit B, brain isoform; V-ATPase subunit B 2; V-type proton ATPase subunit B; Vacuolar ATP synthase subunit B brain isoform; Vacuolar H+ ATPase 56,000 subunit; Vacuolar H+ ATPase 56000 subunit; Vacuolar H+ATPase B2; Vacuolar proton pump subunit B 2; VAT B2; VATB 2; VATB; VATB2. |
| 保存条件 ： |
Store at -20°C for one year. Avoid repeated freeze-thaw cycles. At 4°C for 2-3 weeks after reconstituted. |
| 标 记 物 ： |
PLLABS公司可供应生物素、辣根过氧化物酶、荧光素标记抗体 |
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产品说明 ： |
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