产品货号Reference:             CAN-FT4-6040
检测方法Kit Type:                Competitive LIA
规格Kit Size:                        96-well break-apart microplate
灵敏度Sensitivity:               1 pg/mL
检测样本Sample Type:        Human serum / 25 μL
校准范围Calibrator Range: 2–80 pg/mL
检测时间Total Assay Time: 90 minutes

背景介绍:
甲状腺素(T4)是主要的甲状腺激素，在血液中几乎完全与载体蛋白结合。然而，只有游离的(未结合的)甲状腺素被认为是生物活性的。甲状腺素的主要携带者是甲状腺素结合球蛋白(TBG)、前白蛋白和白蛋白。与总甲状腺素水平相比，游离甲状腺素(FT4)水平与临床状态的相关性更好。与平衡透析法和超渗滤法相比，DbC游离法T4测定是一种快速、简便的一步竞争液相免疫分析方法，且操作繁琐、耗时，是一种快速、简便的一步竞争LIA分析方法。该系统使用了高特异性的单克隆抗体和一种非模拟示踪剂，实验证明该示踪剂没有fi不能与TBG和白蛋白结合的信号。正常甲状腺组织中游离T4浓度为7~22pg/mL。甲状腺功能减退症患者游离T4水平降低，而甲状腺功能亢进症患者游离T4水平升高。该方法有时与其他甲状腺试验一起用于体外诊断和评估接受甲状腺治疗的患者。
Thyroxine (T4), the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. However, only the free (unbound) fraction of thyroxine is considered to be biologically active. The main carriers of thyroxine are thyroxine-binding globulin (TBG), pre-albumin and albumin. The measurement of free thyroxine (fT4) levels correlate better with the clinical status than total thyroxine levels.
The DBC free T4 assay is a one step competitive LIA system that is rapid and easy to perform compared to equilibrium dialysis and ultrafi ltration methods, which are cumbersome and time-consuming. This system employs a highly specifi c monoclonal antibody and a non-analog tracer that was proved experimentally to have no signifi cant binding to TBG and albumin.
In the euthyroid, normal population the free T4 concentration is 7–22 pg/mL. The level of free T4 is decreased in hypothyroidism while in thyrotoxic patients the level of free T4 is increased.
This assay is used at times with other thyroid tests for in vitro diagnostic purposes and for assessing patients who are receiving thyroid treatments (follow-up).

注：本产品仅用于科研用途，不能直接用于人体或临床诊断使用。