产品货号Reference:            CAN-APN-5000
检测方法Kit Type:               Sandwich ELISA
规格Kit Size:                        96-well break-apart microplate
灵敏度Sensitivity:                0.06 ng/mL
检测样本Sample Type:         Human serum or plasma / 20 μL
检测范围Calibrator Range:  2–50 ng/mL
检测时间Total Assay Time:  105 minutes

背景介绍：

脂联素ELISA的原理是两步夹心酶联免疫分析。该方法使用了两种高特异性的单克隆抗体：一种是将一种针对脂联素的单克隆抗体固定在微板上，另一种是针对不同的脂联素表位的单克隆抗体与生物素结合。在第一步中，样品和标准品中的脂联素结合到固定抗体和生物素化抗体上，从而形成一个三明治复合物。未结合的生物素化抗体通过洗涤去除。在第二步中，加入链霉亲和素-HRP，它能特异性地与生物素化抗体结合。通过洗涤除去未结合的链霉亲和素HRP。接下来，添加酶底物(TMB)。酶促反应的显色强度与脂联素的浓度成正比。酶的反应通过加入停止溶液而终止。在微孔板阅读器上测量了450 nm处的吸光度。样品和对照品中的脂联素浓度可以从标准曲线的图解中计算出来。
The principle of the adiponectin ELISA is a two-step sandwich enzyme immunoassay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for adiponectin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of adiponectin is conjugated to biotin. During the first step, adiponectin present in the samples and standards is bound to the immobilized antibody and to the biotinylated antibody, thus forming a sandwich complex. Unbound biotinylated antibody is removed by a washing. In the second step, streptavidin-HRP is added, which binds specifically to bound biotinylated antibody. Unbound streptavidin-HRP is removed by washing. Next, the enzyme substrate (TMB) is added. The colour intensity of the enzymatic reaction is directly proportional to the concentration of adiponectin. The enzymatic reaction is terminated by the addition of stopping solution. The absorbance is measured on a microplate reader at 450 nm. The concentration of adiponectin in samples and controls can be calculated from of a plot of the standard curve, either graphically or by using immunoassay software.