产品货号Reference:              CAN-RT3-100
检测方法Kit Type:                 Competitive ELISA
规格Kit Size:                         96-well break-apart microplate
灵敏度Sensitivity:                 0.009 ng/mL
检测样本Sample Type:          Human serum or plasma / 25 μL
检测范围Calibrator Range:   0.02–2 ng/mL
检测时间Total Assay Time:  105 minutes

背景介绍：
DBC的RT3 ELISA试剂盒采用竞争性酶联免疫分析方法，其抗原(存在于校准器、对照和病人标本中)与生物素标记的抗原(RT3-生物素结合物)竞争，以获得包被在微孔板上的有限数量的抗体。孵育1小时后，第一次洗涤，去除未粘结的材料，加入链霉亲和素-HRP结合物，孵育30分钟。在第二次洗涤之后，添加TMB基板。酶反应通过加入停止溶液而终止，在停止溶液的基础上用微孔板读取器测量颜色强度。显色强度与样品中rT3的浓度成反比。与样品同时运行的一套试剂盒校准器用于绘制校准曲线，并测定样品和对照品中rT3的浓度。
The DBC rT3 ELISA is a competitive enzyme immunoassay, where the antigen (rT3 present in calibrators, controls and patient samples) competes with a biotin-labelled antigen (rT3-Biotin conjugate) for a limited quantity of antibody which is coated on the microplate wells. After one hour incubation followed by the first washing, unbound materials are removed and a Streptavidin-HRP conjugate is added and incubated for 30 minutes. Following a second washing, the TMB substrate is added. The enzymatic reaction is terminated by addition of the stopping solution, upon which the colour intensity is measured with a microplate reader. The colour intensity is inversely proportional to the concentration of rT3 in the sample. The set of kit calibrators that are run simultaneously with the samples is used to plot a calibration curve and determine the concentration of rT3 in samples and controls.

注：本产品仅用于科研用途，不能直接用于人体或临床诊断使用。